osteogenic induction medium stemcell catalog Search Results


osteogenic induction media (om) containing ascorbate, dexamethasone, β-glycerophosphate  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc osteogenic induction media (om) containing ascorbate, dexamethasone, β-glycerophosphate
Osteogenic Induction Media (Om) Containing Ascorbate, Dexamethasone, β Glycerophosphate, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/osteogenic induction media (om) containing ascorbate, dexamethasone, β-glycerophosphate/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
osteogenic induction media (om) containing ascorbate, dexamethasone, β-glycerophosphate - by Bioz Stars, 2026-06
90/100 stars
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osteogenic induction medium stemcell catalog #05465  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc osteogenic induction medium stemcell catalog #05465
hUCMSCs acquisition and identification, and large‐scale expansion in hollow fiber bioreactor. (a) Schematic diagram of acquisition and identification, and large‐scale expansion of hUCMSCs, which were first expanded to 10 7 –10 8 in the culture flask, then inoculated into the hollow fiber bioreactor until amplified to 10 9 –10 10 ; (b) hUCMSCs were isolated by the tissue block method; (c) P5‐generation hUCMSCs morphology under an optical inverted microscope. (d) hUCMSCs differentiated into fat and stained with Oil red O (×200). (e) <t>Osteogenic</t> differentiation of hUCMSCs stained with alizarin red (×200). (f) Cartilage differentiation of hUCMSCs stained with safranin O (×200). (g) Flow cytometry showed that hUCMSCs highly expressed CD73, CD90, and CD105 (both ≥ 95%) but did not express CD34, CD45, CD19, CD11b, or HLA‐DR (both ≤ 5%); (h) The results of live and dead staining of hUCMSCs in the bioreactor; (i) hUCMSCs in the bioreactor under a light microscope; (j) Hollow‐fiber superfine structure in the bioreactor. (k) Adhesion of hUCMSCs to hollow fiber in the bioreactor. (l–m) Changes in GLU, Lac, and PO 2 in the bioreactor during hUCMSCs culture in (l) Group B and (m) Group C. GLU, glucose; Lac, lactate; PO 2 , oxygen partial pressure. Scale bar 200 μm.
Osteogenic Induction Medium Stemcell Catalog #05465, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/osteogenic induction medium stemcell catalog #05465/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
osteogenic induction medium stemcell catalog #05465 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
osteogenic growth medium cat. no. 05465  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc osteogenic growth medium cat. no. 05465
Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, <t>osteogenic</t> growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.
Osteogenic Growth Medium Cat. No. 05465, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/osteogenic growth medium cat. no. 05465/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
osteogenic growth medium cat. no. 05465 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


hUCMSCs acquisition and identification, and large‐scale expansion in hollow fiber bioreactor. (a) Schematic diagram of acquisition and identification, and large‐scale expansion of hUCMSCs, which were first expanded to 10 7 –10 8 in the culture flask, then inoculated into the hollow fiber bioreactor until amplified to 10 9 –10 10 ; (b) hUCMSCs were isolated by the tissue block method; (c) P5‐generation hUCMSCs morphology under an optical inverted microscope. (d) hUCMSCs differentiated into fat and stained with Oil red O (×200). (e) Osteogenic differentiation of hUCMSCs stained with alizarin red (×200). (f) Cartilage differentiation of hUCMSCs stained with safranin O (×200). (g) Flow cytometry showed that hUCMSCs highly expressed CD73, CD90, and CD105 (both ≥ 95%) but did not express CD34, CD45, CD19, CD11b, or HLA‐DR (both ≤ 5%); (h) The results of live and dead staining of hUCMSCs in the bioreactor; (i) hUCMSCs in the bioreactor under a light microscope; (j) Hollow‐fiber superfine structure in the bioreactor. (k) Adhesion of hUCMSCs to hollow fiber in the bioreactor. (l–m) Changes in GLU, Lac, and PO 2 in the bioreactor during hUCMSCs culture in (l) Group B and (m) Group C. GLU, glucose; Lac, lactate; PO 2 , oxygen partial pressure. Scale bar 200 μm.

Journal: Bioengineering & Translational Medicine

Article Title: A simple and efficient strategy for cell‐based and cell‐free‐based therapies in acute liver failure: hUCMSCs bioartificial liver

doi: 10.1002/btm2.10552

Figure Lengend Snippet: hUCMSCs acquisition and identification, and large‐scale expansion in hollow fiber bioreactor. (a) Schematic diagram of acquisition and identification, and large‐scale expansion of hUCMSCs, which were first expanded to 10 7 –10 8 in the culture flask, then inoculated into the hollow fiber bioreactor until amplified to 10 9 –10 10 ; (b) hUCMSCs were isolated by the tissue block method; (c) P5‐generation hUCMSCs morphology under an optical inverted microscope. (d) hUCMSCs differentiated into fat and stained with Oil red O (×200). (e) Osteogenic differentiation of hUCMSCs stained with alizarin red (×200). (f) Cartilage differentiation of hUCMSCs stained with safranin O (×200). (g) Flow cytometry showed that hUCMSCs highly expressed CD73, CD90, and CD105 (both ≥ 95%) but did not express CD34, CD45, CD19, CD11b, or HLA‐DR (both ≤ 5%); (h) The results of live and dead staining of hUCMSCs in the bioreactor; (i) hUCMSCs in the bioreactor under a light microscope; (j) Hollow‐fiber superfine structure in the bioreactor. (k) Adhesion of hUCMSCs to hollow fiber in the bioreactor. (l–m) Changes in GLU, Lac, and PO 2 in the bioreactor during hUCMSCs culture in (l) Group B and (m) Group C. GLU, glucose; Lac, lactate; PO 2 , oxygen partial pressure. Scale bar 200 μm.

Article Snippet: According to the manufacturer's instructions, adipogenic induction medium (STEMCELL, Catalog #05412), osteogenic induction medium (STEMCELL, Catalog #05465), and chondrogenic induction medium (STEMCELL, Catalog #05455) were used to culture hUCMSCs.

Techniques: Amplification, Isolation, Blocking Assay, Inverted Microscopy, Staining, Flow Cytometry, Light Microscopy

Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression, Incubation, Expressing, Control, Concentration Assay, Binding Assay

Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Concentration Assay

Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Binding Assay, Concentration Assay

Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

Journal: International Journal of Molecular Medicine

Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

doi: 10.3892/ijmm.2020.4556

Figure Lengend Snippet: Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control